[Frontiers in Bioscience 10, 83-87, January 1, 2005] 83 CHARACTERIZATION OF MEMBRANE-ASSOCIATED SUBSTRATES OF Ca-DEPENDENT KINASES IN ASTROCYTES
نویسندگان
چکیده
Ljubisa Vitkovic , Shigeru Maeda 2 and Vincent J. Aloyo 3 1 Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, 2Integrative Neural Immune Program, National Institute of Mental Health, National Institutes of Health, Bethesda, MD 20892-1284, Department of Pharmacology, Medical College of Pennsylvania, Philadelphia, PA 19129
منابع مشابه
Identification of myristoylated alanine-rich C kinase substrate (MARCKS) in astrocytes.
We have characterized membrane-associated substrates of Ca2+-dependent kinases in primary rat astrocytes by in vitro phosphorylation, 2-dimensional gel electrophoresis and autoradiography. The most prominent among these were three acidic, protein kinase C (PKC) substrates. These are important because they likely transduce cytokine and other neuro-immune modulatory signals mediated by PKC. We no...
متن کاملTracking functions of cGMP-dependent protein kinases (cGK).
The options available for distinguishing the effects of cGMP mediated by cGK versus those mediated by other cGMP targets are discussed and evaluated. These include the unnecessary but often sole reliance on synthetic, small-molecule activators and inhibitors of cGK which are increasingly recognized as deficient in specificity. Other important adjunct options include cGK overexpression using ade...
متن کاملGene knockouts that cause female infertility: search for novel contraceptive targets.
The gene knockout technology has revolutionized the fertility/infertility field. It has revealed several essential previously undiscovered molecules, new insights and novel mechanisms involved in steps of the fertility cascade in females. Using database and literature search, knockouts of at least 83 genes were discovered that demonstrated an effect on fertility of female mice. These effects ra...
متن کاملTranscriptomic analysis reveals pH-responsive antioxidant gene networks.
Reactive oxygen species (ROS) are produced in different physiological conditions. In response to ROS imbalance cells activate oxidative stress defenses, which include more than 60 antioxidant genes. It has been suggested that gene products associated with ROS detoxification can work coordinately, acting as an antioxidant-defense network. However, the functional overlap among oxidative stress de...
متن کاملRegulation of CDC14: pathways and checkpoints of mitotic exit.
Progression of the mitotic cell cycle is driven by fluctuations of the cyclin-dependent kinase (Cdk) activities. Entry into mitosis is promoted by the elevated activity of Cdk1 associated with B-type cyclins. Conversely, exit from mitosis requires the inactivation of Cdk1 and the dephosphorylation of at least a subset of Cdk1 substrates. The Cdc14 family of phosphatases antagonizes the action o...
متن کامل